Device

Part:BBa_K1172503:Design

Designed by: Lukas Rositzka   Group: iGEM13_Bielefeld-Germany   (2013-08-28)


Outer membrane porin OprF from Pseudomonas fluorescens with Plac and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 990
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1382
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 903


Design Notes

  • Classical [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Suffix_Insertion Suffix-Insertion] of the Biobrick BBa_K1172501 into the Backbone of the BioBrick BBa_K04500.

Source

Pseudomonas fluorescens Migula 1895 genomic DNA

DSMZ No.: 7355

References